uM workstation concept

  • Problem: Micropipette based research techniques provide invaluable data, but are difficult and slow to perform
  • Solution: uM workstations automate laborious steps and workflows to facilitate deployment and to improve throughput
  • Technical concept: accurate and synchronized sample, pipette tip and objective positioning in closed-loop control with the pipette-cell interface pressure and recorded electrical signals
  • Applications: in vitro and in vivo patch clamp, single cell microinjections and cell picking for genetic analysis; often in sync with imaging and opto-stimulation

Affordable and scalable – from supercharged classic setups to automated workflows

We believe automation is the future also in electrophysiology and imaging, but we also understand that going all the way is not always feasible or even beneficial. Automating specific laborious step or partial workflow of an experiment is often enough for significant throughput improvement. We have designed uM workstations to be affordable and modular to facilitate wide adoption (see our webstore for pricing).

Sensapex products are based on proprietary and patented technologies, which integrate together via fully digital and parallel control architecture. This provides smallest control latencies, unlimited scalability and most versatile PC control interface (Ethernet; open-source Sensapex SDK).

uM workstations are often used with ACQ4 open-source software platform, which has growing user base for the automated techniques. We are active at the Github and at the dedicated Slack channel for the uM workstations and automated techniques. Join us and growing user community to implement latest technology to your experiments!

Examples of unique features – please contact us to discuss your requirements

    • uMp-TSC touch screen unit and uMp-RW3 rotary wheel controllers provide stand-alone control interface for all Sensapex products. In addition to basic operation, many usability improving features and system level functionalities are available through quick buttons for convenient use.
    • 1-click automated pipette cleaning from uMp-TSC touch screen for immediate re-use with another cell
    • Camera image based sample and pipette tip control. Click your target from image to place it to desired location and/or to drive pipette tip to it accurately. The uM workstations know in real time where specified targets and pipette tips are for accurate automation.
    • Automated whole cell patch clamp. Operator clicks on the desired cells from camera image and selects protocol – uM workstation performs experiments

What does uM workstation delivery include?

Depending on your needs, the uM workstation delivery may include all or some of the products, software packages and services listed below. All products are delivered with their standard 2 years limited warranty (extensions available).

  • uMs microscope with motorized xy-stage, focus and objective changer (or single objective holder)
  • uMp micromanipulator(s), touch screen unit and rotary wheel controller
  • uMc automated pressure control products
  • Free, open-source software development kit (SDK) with application examples (available at Github)
  • For automated uM workstations: Sensapex products, PC installed with ACQ4, camera (+other agreed 3rd party products), system integration and testing before shipment
  • On-site or remote installation is available with per day or per hour rates (possible travelling expenses charged separately)

Standard delivery does not include any guarantees or free-of-charge work to implement your experiments, but we are more than happy to discuss your needs and offer a turnkey project.

Classic patch clamp setup – superior stability and accuracy

    • uMs-FN1 microscope with motorized focus
    • uMs-OBJ-1 1-position objective holder
    • uMs-FXY fixed physiology stage
    • uMp-3 ZERO DRIFT micromanipulator (incl. accessories)
    • uMp stand-alone controllers
    • Standard Nikon FN1 products for patch clamp experiments (incl. transmitted light illumination and oblique contrast method)

See example system in webstore

ItemDescriptionQty
uMs-FN1Sensapex uMs-microscope with motorized, closed-loop controlled focus, Nikon FN-1 based
1
uMs-OBJ-11-position objective holder1
uMs-MCUMotor controller supporting closed-loop control of up to 4 motorized axes
(xy-stage, focus and objective exchanger)
1
uMs-FXYUltra-stable, fixed xy-stage that supports plug-and-play installation of 8 uMp-3
manipulators
1
uMp-3Triple axis manipulator, simple bolt-on mounting base. Shipped as right-handed
unless left-handed is specified; customer reconfigurable at any time
1
uMp-EXMAdd-on rotate, flip & slide module1
uMp-DVTDove-tail type headstage adapter1
uMp-TSCTouch screen control unit incl. charger and ethernet cable; supports direct
connection of 2 manipulators, 2 rotary wheel and PC
1
uMp-RW3Rotary wheel remote interface with 3 wheels1
uM-NikonStandard Nikon FN1 products for patch clamp experiments (incl. transmitted light illumination and oblique contrast method)1

Dual patch clamp from cultured cells – sample and pipette tip automation ready

  • uMs-FN1 microscope with motorized focus
  • uMs motorized xy-stage
  • uMs motorized objective changer
  • 2 pcs uMp-3 ZERO DRIFT micromanipulators (incl. accessories)
  • uMp stand-alone controllers
  • Standard Nikon FN1 products for patch clamp experiments (incl. transmitted illumination and DIC contrast method)
  • Hamamatsu ORCA Spark camera
  • Pre-installed computer, system integration and testing before shipment

See example system in webstore

ItemDescriptionQty
uMs-FN1Sensapex uMs-microscope with motorized, closed-loop controlled focus, Nikon FN-1 based
1
uMs-MXYMotorized, closed-loop controlled xy-translator for upright and inverted
microscopes (excl. controller and stage)
1
uMs-FXYUltra-stable, fixed xy-stage that supports plug-and-play installation of 8 uMp-3
manipulators
1
uMs-BREPlatform to install uMs-AXE over the motorized uMs-AXE-MXY XY-translator1
uMs-OBJ2-position objective changer for the AxioExaminer1
uMs-MCUMotor controller supporting closed-loop control of up to 4 motorized axes
(xy-stage, focus and objective exchanger)
1
uMp-3Triple axis manipulator, simple bolt-on mounting base. Shipped as right-handed
unless left-handed is specified; customer reconfigurable at any time
2
uMp-DVTDove-tail type headstage adapter2
uMp-EXMAdd-on rotate, flip & slide module2
uMp-TSCTouch screen control unit incl. charger and ethernet cable; supports direct
connection of 2 manipulators, 2 rotary wheel and PC
1
uMp-RW3Rotary wheel remote interface with 3 wheels1
uM-NikonStandard Nikon FN1 products for patch clamp experiments (incl. transmitted illumination and DIC contrast method)1
uM-HAM-C11440-36UHamamatsu ORCA Spark1
uM-PCPre-installed computer1

Quad slice patch clamp – automated patch clamp and wide-field imaging ready

    • uMs-FN1 microscope with motorized focus
    • uMs motorized xy-stage
    • uMs motorized objective changer
    • 4 pcs uMp-3 ZERO DRIFT micromanipulators (incl. accessories)
    • uMc automated pressure control products
    • uMp stand-alone controllers
    • Standard Nikon FN1 products for patch clamp experiments (incl. transmitted illumination and IR-DIC contrast method)
    • Hamamatsu ORCA Flash 4.0 LT PLUS camera package
    • Pre-installed computer, system integration and testing before shipment

See example system in webstore

ItemDescriptionQty
uMs-FN1Sensapex uMs-microscope with motorized, closed-loop controlled focus, Nikon FN-1 based
1
uMs-MXYMotorized, closed-loop controlled xy-translator for upright and inverted
microscopes (excl. controller and stage)
1
uMs-FXYUltra-stable, fixed xy-stage that supports plug-and-play installation of 8 uMp-3
manipulators
1
uMs-BREInstallation platform for uMs-FN1 micrscope1
uMs-OBJMotorized 2-position objective changer1
uMs-MCUMotor controller supporting closed-loop control of up to 4 motorized axes
(xy-stage, focus and objective exchanger)
1
uMp-3Triple axis manipulator, simple bolt-on mounting base. Shipped as right-handed
unless left-handed is specified; customer reconfigurable at any time
4
uMp-EXMAdd-on rotate, flip & slide module4
uMp-DVTDove-tail type headstage adapter4
uMp-HUB1 to 4 HUB with extra battery2
uMc-PPC44 channel pressure controller for automated pipette cleaning1
uMc-PRF-VACVacuum generating pressure pre-regulation & filter unit, requires min. +400 kPa high pressure1
uMc-INS-PMChamber insert for pipette cleaning with Warner PM-1 chamber platform1
uMp-TSCTouch screen control unit incl. charger and ethernet cable; supports direct
connection of 2 manipulators, 2 rotary wheel and PC
1
uMp-RW3Rotary wheel remote interface with 3 wheels1
uM-NikonStandard Nikon FN1 products for patch clamp experiments (incl. transmitted illumination and IR-DIC contrast method)1
uM-HAM-C11440-42U30Hamamatsu ORCA Flash 4.0LT PLUS1
uM-PCPre-installed computer1

Sensapex products are proprietary and patented (US 9,138,892, US 9,662,783, EP 2 776 215, US 10,427,292; EP 09851052.2, EP15906190.2, US 15/765,608, EP: 15906189.4). Automated patch clamp (US 9,498,293 and US 9,668,804) and pipette cleaning (US 15/232,770 and EP 16836035.2) products are manufactured under licenses from GTRC and MIT, U.S.A.