Behaviorally relevant sensory-motor processing is conducted by large neuronal populations across the brain. An ideal experiment to study this would involve recording brain-wide neural activity of each neuron with sub-millisecond temporal resolution. Although still unachievable, development of extracellular recording techniques has enabled scaling the experimental yield from few (single channel metal microelectrodes) to few tens (wire tetrodes) to few hundreds (silicon probes) of simultaneously recorded neurons. This application note describes the latest technology improvement, called Neuropixel probe, that enables yet another order of magnitude scaling to few thousands of simultaneously recorded neurons.
• Use of eight Neuropixels probes allows recording from up to 3000 neurons simultaneously in awake mice
• These recordings revealed complex population activity and widespread behavioral representations
• Future experiments will be able to utilize this approach to understand previously inaccessible aspects of neural coding and the midbrain in awake mice.